Decellularized cell sheet constructs for periodontal regeneration

Cell Sheet Engineering for Periodontal Regeneration

Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration

Periodontal regeneration is an important part of regenerative medicine, with great clinical significance; however, the effects of nanotopography on the functions of periodontal ligament (PDL) stem cells (PDLSCs) and on PDLSC sheet based periodontal regeneration have never been explored. Titania nanotubes (NTs) layered on titanium (Ti) provide a good platform to study this. In the current study,...

Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration

Engineering three-dimensional (3D) vascularized constructs remains a challenge due to the inability to form rich microvessel networks. In this study we engineered a prevascularized 3D cell sheet construct for tissue regeneration using human bone marrow-derived mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells as cell sources. hMSCs were cultured to form a thick cell shee...

Decellularized bone matrix grafts for calvaria regeneration

Decellularization is a promising new method to prepare natural matrices for tissue regeneration. Successful decellularization has been reported using various tissues including skin, tendon, and cartilage, though studies using hard tissue such as bone are lacking. In this study, we aimed to define the optimal experimental parameters to decellularize natural bone matrix using 0.5% sodium dodecyl ...

Periodontal Regeneration: Promising and Challenging for Periodontal Complex Regeneration

Periodontitis is one of the most prevalent infectious diseases in human beings, characterized by irreversible destruction on tooth supporting tissues such as alveolar bone, Periodontal Ligaments (PDL) and cement. The pathogenesis involves a complex interaction between the host’s immune response to microbial colony localized in periodontal part, and modifying host factors, including smoking, gen...